GS Caltex has filed a patent for a shuttle plasmid that can replicate in both Clostridium and Escherichia coli. The plasmid includes a nucleotide sequence for replication in E. coli, a replication protein region derived from pUB110 plasmid, and an expression terminator sequence of a gene. GlobalData’s report on GS Caltex gives a 360-degree view of the company including its patenting strategy. Buy the report here.
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According to GlobalData’s company profile on GS Caltex, bioethanol production GMOs was a key innovation area identified from patents. GS Caltex's grant share as of June 2023 was 1%. Grant share is based on the ratio of number of grants to total number of patents.
A shuttle plasmid replicable in clostridium and e. coli
A recently filed patent (Publication Number: US20230183715A1) describes a shuttle plasmid that can replicate in both Clostridium and Escherichia coli. The shuttle plasmid includes a nucleic acid sequence of a replication origin replicable in Escherichia coli, a nucleic acid sequence for encoding a replication protein region derived from a pUB110 plasmid, and an expression terminator sequence of a gene. Additionally, the shuttle plasmid may include an antibiotic resistant gene expressed in both Clostridium and Escherichia coli, with a preference for a chloramphenicol resistant gene. The size of the shuttle plasmid ranges from 3000 bp to 4000 bp. The expression terminator sequence is a nucleic acid sequence of a transcription terminator of a gene for encoding acetoacetate decarboxylase. Importantly, the shuttle plasmid is replicable in both Clostridium and Escherichia coli without replacing an antibiotic resistant gene.
The patent also describes a method for producing a recombinant microorganism using the shuttle plasmid. The method involves preparing the shuttle plasmid as described above, introducing at least one gene into the shuttle plasmid to produce a first recombinant shuttle plasmid, and introducing the first recombinant shuttle plasmid into a microorganism. The genes that can be introduced into the shuttle plasmid include those encoding xylose kinase, xylulose isomerase, alcohol/aldehyde dehydrogenase, and coenzyme A transferase. The microorganism used in the method has an acetyl coenzyme A biosynthetic pathway and a butyryl coenzyme A biosynthetic pathway, and may also include a gene for encoding alcohol/aldehyde dehydrogenase and a gene for encoding coenzyme A transferase. The method may further involve introducing a second recombinant shuttle plasmid into the microorganism, which can include the same gene as the first recombinant shuttle plasmid or a different gene. The resulting recombinant microorganism can be used to obtain a fermentation product by culturing the microorganism and harvesting the fermentation product.
In summary, the patent describes a shuttle plasmid that can replicate in both Clostridium and Escherichia coli, allowing for the production of recombinant microorganisms with specific genes of interest. The method outlined in the patent provides a way to introduce these genes into the shuttle plasmid and subsequently into the microorganism, enabling the production of fermentation products. This technology has potential applications in various fields, including biotechnology and industrial fermentation processes.
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